Fig. 2. Quantification of early-born cells in the E18.5 mutant and wild-type retinas and late-born cells in retinal explants cultured for 7 days. (A) Mutant retinas featured a significant 33.76% reduction in the number of BRN3b-expressing RGCs in the mutant retinas compared with wild-type retinas (asterisk). No significant differences in the number of PAX6-expressing amacrine or NF165-expressing horizontal cells were identified. (B) Late-born retinal cell classes not identifiable in E18.5 retinas were quantified in 7-day-old explant cultures of paired mutant and wild-type retinas collected at E18.5. No significant differences in the number of rods, cones, Müller glia or bipolar interneurons as identified by Rho4D2, peanut agglutinin, glial fibrillary acidic protein (GFAP), and CHX10 immunoreactivity, respectively, were found (rods t=1.03, P>0.05, n=3; cones t=1.26, P>0.05, n=3; Müller glia t=0.64, P>0.05, n=3; bipolar cells t=0.79, P>0.51, n=3). PNA, peanut agglutinin.