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Fig. 3. Reduced optic nerve thickness in Dlx1/Dlx2 mutant eyes. (A,B) Wild-type (A) and Dlx1/Dlx2 mutant (B) optic nerves. Three measurements were made of the optic nerve as it exits the retina (optic nerve head) using the boundary between the GCL and NBL (A, top arrow), the outer retinal limit (A, bottom arrow) and at a point equidistant between these markers (A, middle arrow). There was a significant 23% decrease in the thickness of the mutant optic nerve (asterisk). Scale bars: 125 µm.