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Fig. 6. Wnt4 and Tgfß1 induce Sema3a in the mandibular and dental mesenchymes. Bright- and dark-field images. (A1-B2) Fgf8 induces Pax9 in the presumptive molar mesenchyme at E10, while no effects on Sema3a expression are observed. (C1-E2) A prominent Sema3a expression is seen around the Wnt4-producing cells in the E10.5 and E11 mandibular mesenchyme and E12 dental mesenchyme explants. (F1,F2) Endogenous expression of Sema3a in E12.5 dental mesenchyme explants. (G1,G2) Tgfß1-releasing bead (arrow indicates the site where the bead was located) stimulates Sema3a expression in the dental mesenchyme area of E12.5 lower jaw mesenchyme explants. (J1-K2) Wnt4 cells stimulate Msx1 in the proximal presumptive molar mesenchyme at E10.5, while no expression is observed around the NIH3T3 cells. (L1-P2) No Sema3a stimulation is seen in the mesenchyme surrounding the Wnt6-producing or control NIH3T3 cells or around the Fgf9- or BSA-soaked beads. Wnt6 cells show some endogenous Sema3a hybridization signal. (H1-I2,Q1-Q2) Bmp4, Fgf4 and Fgf9 induce the expression of target genes in control explants. Scale bars: 100 µm. c, cell cluster.