Fig. 1. Gli1 and Shh gene expression in the SVZ. (A,C) Expression of Shh mRNA in the lateral wall of the lateral ventricles (LV) of adult mice. At high magnification, Shh expression is clearly detected in SVZ cells (C). (B,D,F-H) Expression of Gli1 mRNA in the lateral wall of the lateral ventricle of adult (B,D) and postnatal (P3; G,H) mice. (E) Control section showing lack of hybridization of Shh antisense RNA probes in the fourth ventricle (4V) of an adult mouse. Sense probe controls gave no signal. (A-H) in situ hybridizations on cross sections. Arrows point to sites of expression. Dorsal is to the top. Scale bar in F: 400 µm for A,F; 200 µm for C-E; G, 150 µm; H, 30 µm.