Fig. 3. Gene expression in single SVZ cells. (A) Schematic representation of the experimental procedure. Individual SVZ cells were randomly harvested with a patch-clamp pipette from fresh living slices, and were used for single cell multiplex RT-PCR. (B) Examples of RT-PCR assays showing gene expression in single SVZ cells. (C) Summary graph showing the expression of GFAP, Gli1 or both in single cells, shown as percentage of the total number of collected cells (n=65).