Fig. 5. Reduced number of newborn interneurons in the adult olfactory bulb after cyclopamine treatment. (A) Experimental procedure. BrdU injections are done during cyclopamine or vehicle treatment. One month post-injection, the number of newborn neurons is quantified after BrdU staining. (B,C,D,E) Photographs of the BrdU staining in the olfactory bulb of vehicle- (B,C) or cyclopamine- (D,E) treated mice. (F) The number of BrdU⁺ cells in the olfactory bulb of cyclopamine-treated mice (open circle, n=5) is significantly reduced in comparison to vehicle-treated mice (filled circle, n=5) along the antero-posterior axis. Error bars=s.e.m.