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Fig. 6. Co-expression of Shh and megalin in the AEP of E10.5 embryos. In-situ hybridization for Shh (A) and immunohistology for megalin (B) on coronal sections of the rostroventral neural tube of wild-type E10.5 embryos identify co-expression in the AEP (arrowheads). The inset highlights localization of megalin protein on the apical surface of neuroepithelial cells.