Fig. 4. Disruption of neural crest migration is correlated with segmentation defects. Whole-mount staining with crestin riboprobe (red) and F59 antibody (green) of bea (A-D; posterior of somite 9) and fss mutants (E-H) at 21 hpf. Streams of neural crest cells (red, A,C,E,G) are present, but less regular than in wild types and the streams show some branching, consistent with abnormal somite shape and size. However, neural crest still migrates generally in the middle of the medial aspect of the myotome in both mutants. (D,H) DIC images of bea and fss mutants, respectively. Scale bar: 20 µm.