Fig. 7. Neural crest migration is disrupted in Hedgehog signaling mutants. Whole-mount staining (A-C,E-G), and DIC image (D,H) of syu (A-D) and smu mutants (E-H) at 21 hpf with crestin riboprobe and F59 antibody. syu mutants have a significant number of slow muscle cells. Consistent with this, neural crest migration is fairly normal, although the streams are less regular than in wild types. By contrast, smu mutants entirely lack slow muscle and neural crest cells are abnormally patterned and stall at the level of the dorsal aspect of the notochord (arrowhead in E). Somite boundaries are shown as broken lines in G. Scale bar: 20 µm.