Fig. 4. Cerebellar-derived neurospheres mainly give rise to astroglia when transplanted to the forebrain. Constitutive EGFP-expressing adult cerebellar-derived neurospheres were transplanted into the neonatal forebrain (P4) and analyzed at P21. The grafted cells mainly remained in close proximity to the injection site (A). GFAP-expressing cells were found in the corpus callosum (B), in the cortex along the needle tract (C) and close to the ventricle (D). TAG1-expressing cells were found close to the graft site (D). Scale bars: 1 mm in A; 50 µm in B1,C1,D1,E1; 20 µm in B2,C2,D2,E2. Although it appears that many of the GFP cells in B2-D2 are not expressing GFAP, this is a result of the different localization of GFP and GFAP in these cells. Reconstruction of optical stacks demonstrates that the GFP cells shown in these figures are largely GFAP positive.