Fig. 3. AprA is present in and is secreted by vegetative cells. (A) Cells growing in shaking culture (vegetative cells), conditioned growth medium, and conditioned starvation buffer were electrophoresed on an SDS-polyacrylamide gel. A western blot of the gel was stained with affinity-purified anti-AprA antibodies. `aprA^-/a15::aprA' indicates aprA^-/actin15::aprA. (B) Growing cells were stained for AprA by immunofluorescence. Scale bar: 20 µm.