Fig. 5. AprA is expressed in vegetative cells and during early development. Ax2 wild-type cells were starved on filters and samples were harvested at the indicated hours after starvation according to Brock et al. (Brock et al., 2002). (A) A Northern blot of RNA extracted from cells harvested at the indicated times in hours after starvation (V indicates vegetative cells) was probed for aprA mRNA. (B) A western blot of whole cell lysates from cells harvested at the indicated times was stained with anti-AprA antibodies.