Fig. 1. Identification of ARF1 and ARF2 T-DNA insertions. (A)
Positions of T-DNA insertions in ARF1 and ARF2. Boxes
represent exons, lines represent non-coding regions, and inverted triangles
indicate T-DNA insertions. The region used for creation of a dsRNAi construct
is indicated by a bar. Arrowheads indicate the locations of primers used to
create probes. (B) Northern blot analysis of ARF gene expression in
T-DNA insertion mutants and dsARF2 plants. RNA was extracted from
10-day-old seedlings.