Fig. 7. Effects of ethylene and cytokinin signaling on arf2-8 plants. (A) Chlorophyll levels in detached leaves incubated in the dark for 8 days. Black bars represent chlorophyll levels just after detachment and white bars represent chlorophyll levels of leaves incubated in the dark for 8 days. Error bars indicate s.d. (n=6). (B) Average position at abscission where position 1 equals the first opened (stage 13) flower and flowers are numbered basipetally. Error bars indicate s.d. (n>20). (C) Northern blot analysis of ethylene-induced gene expression of arf2-8 plants. Six-week-old plants were incubated in airtight containers with either 10 ppm ethylene or air. RNA was extracted from whole rosettes. (D) Chlorophyll levels in cytokinin-treated detached leaves. Detached leaves were floated on water supplemented with 100 nM 6-benzyladenine or on a DMSO mock control. White, Columbia; cross hatched, Columbia+BA; black, arf2-8; hatched, arf2-8+BA. Error bars indicate s.d. (n=6).