Fig. 8. Phenotypes of arf2-8 nph4-1 arf19-4 plants. (A) Chlorophyll levels in detached leaves incubated in the dark for 8 days. Black bars represent chlorophyll levels just after detachment and white bars represent chlorophyll levels of leaves incubated in the dark for 8 days. Error bars indicate s.d. (n=6). (B) Average position at abscission where position 1 equals the first opened (stage 13) flower and flowers are numbered basipetally. Error bars indicate s.d. (n=25). (C) Northern blot analysis of NPH4/ARF7 and ARF19 expression in senescing leaves. Leaves from Columbia and arf2-8 plants were detached, placed in the dark for various time periods and used for mRNA extraction.