Fig. 2. In-situ hybridization for Sf1 on transverse sections (dorsal, top) of an E17.5 wild-type embryo (A) and an Sf1^–/– littermate (B) at the axial level of the adrenal gland. In-situ hybridization for NF mRNA (blue) and TH immunofluorescence staining (red overlay) have been performed on a consecutive section of the wild-type (C; E, higher magnification) and the Sf1^–/– embryo (D; F, higher magnification). Note that in the Sf1^–/– embryo TH⁺/NF^– `adrenal' chromaffin cells are still present. Scale bars: 100 µm. a, dorsal aorta; srg, suprarenal ganglion.