Fig. 8. (A) Quantification of SOX10-ir, TH-ir and PHOX2B mRNA-expressing cells in the region of the developing adrenal gland in E12.5 wild-type and Sf1 mutant mice. The precise demarcations of the region analysed are described in the text. Every fifth section was counted. Four animals were analysed per group. Data are presented as mean±s.e.m. (B,C) TH (red) and SOX10 (green) double-immunolabelling on transverse sections (dorsal, top) through the region of the developing adrenal gland in wild-type and Sf1^–/– mouse embryos. The area in which cells were counted is demarcated.