Fig. 5. SDN-1 expression in neural and hypodermal tissue. (A-F) Expression pattern of the translational Psdn-1::sdn-1::gfp reporter. (A') SDN-1::GFP is broadly expressed in the three-fold embryo, and is particularly strong in the pharynx (pha) and the motoneurons (mn) of the VNC. (B) In L1 larvae, expression is seen in the VNC (arrow) and around the seam cells (arrowhead). SDN-1::GFP expression is also visible in embryonic DD commissures (out of focal plane in B). (C) During later larval stages, SDN-1::GFP is predominantly found in the nervous system. The nerve ring (nr), and the VNC motoneurons and commissures (com) show the highest expression levels (arrowheads; B,F), but the reporter is also present in touch neurons (arrow; E) and other sensory neurons in the head (arrow; F). SDN-1::GFP can also be detected at a lower level in the body wall hypodermis (arrowhead; D); expression is stronger in hypodermal tissue in the tail (arrow; C).