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Fig. 3. GCM/GCM2 regulates both the number of lz+ progenitors and their subsequent differentiation. (A,B) gcm/gcm2 restricts lz activation in the prohemocytes. Ventral views of lacZ (dark purple) and gcm (red) transcripts in stage 7 lz-lacZ (A) and Df(2L)200;lz-lacZ (B) embryos. (C-F) gcm/gcm2 inhibits the differentiation of the lz+ progenitors into crystal cells. Side views of stage 13 lz-gal;uas-lacZ (C,E) and lz-gal4;Df(2L)200;uas-lacZ (D,F) embryos showing lacZ (dark purple) and gcm (blue) transcripts (C,D), or ß-gal (green) and DoxA3 (red) expression (E,F). (G,H) The lack of gcm/gcm2 induces an increase in the number of crystal cells even in the absence of cell proliferation. Side view of DoxA3 expression in stg2 (G) and Df(2L)200;stg2 (H) stage 13 embryos.