Fig. 3. Maintenance of early pancreatic progenitors in the absence of ß-catenin. Three-color confocal immunofluorescence for ß-catenin (A,A'), Pdx1 (B,B') and glucagon (C,C') in E11.5 dorsal pancreatic buds. The relative distribution of Pdx1⁺ and glucagon⁺ cells is normal in regions of the PBKO pancreas lacking ß-catenin (A'-D'). Immunohistochemical staining for pan-ß-catenin or Pdx1 in near-adjacent sections of E13.5 wild-type (E,F) or PBKO (E',F') pancreata reveals normal expression of Pdx1 in ß-catenin-deficient epithelia. Pancreata are counterstained with Hematoxylin (blue) in F,F'. Arrowhead in E' indicates background staining that outlines ß-catenin-deficient epithelia.