Fig. 5. JAG promotes FUL and SHP expression redundantly with FIL and YAB3. (A-D) FUL GUS reporter activity in the gynoecium using the ful-1 enhancer trap line. (E-N) SHP2::GUS reporter activity in stage 15-16 fruit (E-J) and stage 12 gynoecia (K-N). (A,B) Whole mount (A) and cross section (B) of FUL GUS staining in a stage 12 jag fil yab3^+/– gynoecium showing reporter activity restricted to small islands corresponding to the valves. (C,D) Whole mount (C) and cross section (D) of FUL GUS staining in a stage 12 jag fil yab3 gynoecium. Reporter activity is completely absent from the valves. (E,F) Frontal (E) and side (F) views of SHP2::GUS expression in wild type. (G) In jag mutant fruit, a small wedge of ectopic reporter activity is detected in the apical region of the valves (black arrowhead). (H) In fil mutant fruit, a stripe of ectopic reporter activity can be seen extending through the center of the valve, overlying the vasculature. (I) In fil yab3^+/– mutant fruit, the intensity of reporter activity in the valves is stronger than in fil single mutants. (J) In jag fil mutant fruit, strong reporter activity is present in a stripe in the valves with weaker staining elsewhere. (K,L) Cross section of a jag fil yab3^+/– gynoecium showing strong ectopic reporter activity in the valves. Using dark-field microscopy, strong GUS staining appears purple, whereas weak staining appears pink/orange. (L) The same section as in K viewed under bright-field microscopy. (M,N) Cross section of a jag fil yab3 gynoecium showing weak reporter activity in the valves (M). Using bright-field microscopy (N) to image the section in M, the loss of SHP2::GUS reporter activity in the valves and valve margins is even more apparent, compared to the strong GUS staining seen in the valve regions of jag fil yab3^+/– gynoecia (L). v, valve; r, replum; ov, ovule and st, style. Scale bars: 200 µm (A,C); 100 µm (B,D); 0.5 mm (E-J); 100 µm (K-N).