Fig. 6. Egfr antigen localization is rapidly altered in Egfr^tsla after temperature shift. Eye discs with mosaic clones homozygous for Egfr^tsla were stained for Egfr antigen. In all figures, anterior is shown to the right. (A,C,E) Posterior region; (B,D,F-H) the furrow. Times and temperatures are indicated left, see text. A granular, non-nuclear stain in all parts of the disc (white arrows) and a strong additional stain in the furrow between specific cells (white arrowheads in B) is observed. Note that rapidly after the shift to 30°C the furrow cell border stain is lost and is replaced by a heavy antigen stain in larger granules. (G,H) Eye discs stained for (G) Armadillo (Arm), and for (H) Hrs (green) and Egfr (red). Note, Armadillo junctional stain persists after temperature shift (arrow in G), but that Egfr and Hrs do not co-localize (white arrowhead in H indicates an Hrs-positive granule and black arrowhead in H indicates an Egfr-positive granule). Scale bar: 5 µm.