Fig. 5. Expression of reelin signalling molecules in GN11- and GnRH neurons. (A) RT-PCR analysis, using GN11 cell mRNA, showed the presence of ApoER2 and Vldlr transcripts (313 bp and 253 bp respectively), but absence of Dab1 (predicted amplicon 79 bp). These results were confirmed by nested PCR experiments (B), yielding the predicted 201 bp and 186 bp products for ApoER2 and Vldlr, but not the expected band of 60 bp for Dab1. (C-E) Double immunohistochemistry experiments showed the localization of ApoER2 (red, D and E) in a small proportion (arrow in E) of GnRH neurons (green, C and E). (F-H) Similar experiments failed to identify Dab1 (red, G and H) in GnRH neurons (green, F and H). n=3 brains. Scale bar: 50 µm.