Fig. 2. Activation of the node/notochord enhancer and CE in Wnt/ß-catenin treated P19 cells. Expression of reporters containing the enhancer (A) or 8 copies of CE (8xCE) (B) at 48 hours after co-transfection of Wnt expression plasmids. Expression of reporters containing the enhancer (C) or 8xCE (D) at 48 hours after co-transfection of stabilized-ß-catenin expression plasmid. (E) Timecourse of reporter activation after co-transfection with stabilized ß-catenin expression plasmid. Luciferase activities were normalized to the activity of 8xCE reporter without ß-catenin at each time point. In Figs 2, 3, and 4, the results of luciferase assays represent the average of two samples with standard deviations.