Fig. 7. Tead regulates NE enhancer activity and notochord development in vivo. Electroporation of control (A,B) and Tead-EnR expression plasmid (C,D) in enhancer-LacZ transgenic mouse embryos. Representative cross-sections of embryos in A and C are shown in B and D, respectively. Green and red represent NE enhancer activity and electroporated cells, respectively. (A,B) Control electroporation did not affect NE expression. (C,D) Tead-EnR repressed NE activity and notochord development. en, endoderm; nc, notochord; nt, neural tube; s, somite. (E) Model of Foxa2 regulation in the node and notochord. In the node, a Wnt signal promotes formation of the Tead-POT complex on the core element of the enhancer. Once the cells migrate from node to notochord, POT disappears from the CE, and Tead together with other transcription factors (gray objects) continue the activation of the enhancer.