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Fig. 8. Tead regulates foxa2 in the zebrafish shield. Expression of foxa2 (A-C) or ntl (D) in shield-stage embryos injected with various RNAs. Injection of EGFP RNA (100 pg) did not alter Foxa2 expression. Injection of VP16-Tead (B; 100 pg) expanded Foxa2 expression (n=102/125), while injection of Tead-EnR (C,D; 25 pg) reduced Foxa2 expression (C; n=26/36) without affecting expression of ntl (D; n=13/13).