Fig. 4. Localization of postplasmic/PEM mRNAs in embryos injected with Hr-POPK-1 MO. (A,B,C, left columns) Embryo injected with control MO. (A,B,C, right columns) Embryo injected with POPK-1 MO. (A) macho-1 localization at the early 8-cell stage (animal views), and late 8-cell stage (posterior views). (B) macho-1 localization at the 2-cell stage (posterior views), 4-cell stage (animal views), 16-cell stage (animal views), and 32-cell stage (animal views). White arrowheads indicate macho-1 mRNA localization. (C) Localization of various Type I postplasmic/PEM RNAs. The probes are indicated on the left side. Scale bars: 100 µm. (D) Semi-quantitative RT-PCR analysis of macho-1, Hr-POPK-1, Hr-ZF1 and Hr-Notch mRNA using ten uninjected embryos (left) and ten embryos injected with POPK-1 MO (right) at the late 8-cell stage. RT+ and RT–, with and without reverse transcriptase.