Fig. 7. Type I postplasmic/PEM RNA localization on isolated cortices of eggs and 8-cell embryos. cER was stained with DiIC₁₈ (3) and emits red fluorescence. Particle with intense red fluorescence in left panels are micelles of DiIC₁₈ (3). In-situ hybridization signals of Hr-POPK-1, Hr-ZF1 and Hr-Wnt-5 are green. (A-D) Fluorescent images of the cortices isolated from unfertilized eggs. (A) cER (left) and Hr-POPK-1 mRNA (middle). The right panel shows a merged image. (B) cER (left), Hr-ZF1 (middle) and a merged image (right). (C) cER (left), Hr-Wnt-5 (middle) and a merged image (right). (D) cER (left), macho-1 (middle) and a merged image (right). White arrowheads in A-C indicate small granules with bright green fluorescence. (E-G) Confocal images of the CAB domain in the posterior cortices isolated from 8-cell stage embryo. (E) Hr-POPK-1 mRNA. (F) Hr-ZF1 mRNA. (G) Hr-Wnt-5 mRNA. Scale bars: 5 µm.