Fig. 8. Confocal images of the cER/mRNA domain in the posterior cortices in uninjected embryo and embryo injected with POPK-1 MO. cER was stained with DiIC₁₈ (3) and emits red fluorescence. In-situ hybridization signal of Hr-PEM1 is green. (A) cER (left) and Hr-PEM1 (middle) in cortex that was isolated from an uninjected embryo at 8-cell stage. The right panel shows a merged image. (B) cER-rich domain and Hr-PEM1-rich domain overlap in cortices isolated from embryo injected with POPK-1 MO. White arrowheads indicate the cER-rich region. cER/mRNA domain is smaller and rounded in embryos injected with POPK-1 MO. (C-E) Live cortices isolated from 2-, 4-, and 8-cell embryos, respectively. (F) cER and Hr-PEM1 mRNA in posterior cortices isolated from 4-cell stage embryo. Hr-PEM1-rich domain overlaps with the cER-rich domain, as shown by the merged image (right). Scale bars: 10 µm.