Fig. 5. Drosophila pds5 mutations cause chromosome segregation and cohesion abnormalities. (A) The panels show sample third instar neuroblast metaphases for the indicated genotypes. For heterozygous pds5^e3/+ mutants, 117 metaphases were scored: 15.4% showed aneuploidy and 12.8% showed precocious sister chromatid separation (PSCS), similar to wild type (Rollins et al., 2004). Fifty-one metaphases were scored for homozygous pds5^e3 mutants: all showed aneuploidy and 65% displayed PSCS (arrows). Thirty-two homozygous pds5^e6 metaphases were scored: 87.5% showed aneuploidy and 93.8% showed PSCS. (B) Predicted structure of the pds5 gene (CG17509). The site of the viable P transposon insertion (P{EPgy2}CG17509^EY06473) used to generate pds5^e3 and pds5^e6 is shown by the red circle. Exons are numbered boxes, with blue indicating the open reading frame.