Fig. 1. Subcellular localization of the WER-GFP. (A) Schematic diagrams of P_WER:GFP and P_WER:WER-GFP gene constructs. The stop codon of the WER was deleted for the translational fusion with the GFP. (B) Complementation of the wer mutation with P_WER:WER-GFP. The wer-1 mutant was transformed with P_WER:WER-GFP. wt, wild type. (C) WER-GFP accumulation in the Arabidopsis root epidermal cells. The wer-1 mutant transformed with P_WER:WER-GFP and the wild-type plant transformed with P_WER:GFP were grown on agarose-solidified media. Fluorescence was observed in the 4-day-old seedlings using confocal microscopy.