Fig. 6. Effect of the WER protein on CPC expression in Arabidopsis. (A,C) Schematic diagrams of the reporter genes and the effectors used in this transient expression assay (A) and stable transgenic study (C). For reporter constructs, several versions of CPC promoters that are 700 bp long (from the translational start site) were inserted upstream of the luciferase gene (A) or ß-glucuronidase gene (C). Each mutant reporter contains mutations at WBSI or WBSII, or both. For the effector construct, the genomic DNA fragment of WER-coding region was inserted downstream of the 35S promoter (A). (B) Importance of WBSI and WBSII (refer to A) in CPC expression in the Arabidopsis protoplast transient expression assay. Protoplasts were transfected with UBQ10-GUS as an internal control, each of the reporters (wild type, M1, M2 or M3) and an effector (WER). (D) Importance of WBSI and WBSII (refer to C) in the proper expression of CPC in the Arabidopsis root epidermis. The seedlings were stained for 24 hours.