Fig. 2. Mitotic progression is affected in the VNC of borr mutant embryos. (A) Counts of mitotic cells, as judged by P-H3 staining and chromatin morphology (see also Fig. 4B,D), in the VNC of wild-type or borr mutant embryos at stage 12 (n=6 or 7, respectively), and at stage 14 (n=12 or 10, respectively). (B,C) Relative frequencies of the four main mitotic stages (see D), expressed in percentages at stage 12 (B) and stage 14 (C). Asterisks in A-C indicate statistical significance (**P<0.0005) of the observed differences. (D) Examples of mitotic cells in which chromatin has been visualised by P-H3 staining, selected to illustrate the four main mitotic stages of wild-type and borr mutant VNC cells. There is abnormal chromatin segregation in the mutant anaphase.