Fig. 3. Subcellular localisation and function of Borr in cultured Drosophila cells. (A-G) Mitotic DmD8 cells transiently expressing GFP-Borr, stained with Hoechst dye and antibodies against ß-tubulin, Aurora B or Incenp, as indicated. (H-M) Kc167 cells, (H) mock-treated or (I-M) treated with borr dsRNA. Staining is with Hoechst dye (blue) and phalloidin (red), and with antibody against ß-tubulin (green). Arrows indicate DNA fragments in the cytoplasm of a multi-nucleate Borr-depleted cell (M).