Fig. 4. Reduced P-H3 levels in VNC cells of borr mutant embryos. (A-D) Ventral views of stage 13 (A,B) wild-type or (C,D) borr mutant embryos, stained with Hoechst dye and antibody against P-H3; projections of z-stacks of confocal sections with identical settings are shown, revealing lower numbers of mitotic cells in the mutant and reduced P-H3 levels (see also Fig. 2A,D). (E-J) Individual mitotic VNC nuclei prior to anaphase from (E-G) wild-type or (H-J) borr mutant stage 13 embryos; arrow in I indicates a mutant nucleus with a degree of chromatin condensation similar to that in wild type (F). There are reduced levels of P-H3 staining in both mutant nuclei (J, compare with G).