Fig. 5. Aurora B and Incenp fail to localise to mitotic spindles in borr mutant imaginal disc cells. Wing discs of late third instar larvae bearing borr mutant clones, triple-stained with Hoechst dye and antibodies against ß-tubulin and (A-E') Aurora B or (F-J') Incenp. Areas containing wild-type or mutant mitotic cells are boxed, and the corresponding individual channels are shown below (B'-E',G'-J', wild-type cells; B-E,G-J, borr mutant cells). Localised Aurora B (C) and Incenp (H) staining is missing in the abnormal borr mutant spindles. Scale bars: in A, 5 µm for A,F; in B, 5 µm for B-E',G-J'.