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Fig. 2. Expression patterns of RALDH1, RALDH2 and RALDH3 during eye development. In situ hybridization using digoxigenin-labeled Aldh1a1 (A,D,G), Aldh1a2 (B,E,H) and Aldh1a3 (C,F,I) antisense riboprobes at E10.5 (A-C), E11.5 (D-F) and E13.5 (G-I). Arrowheads (C,F) indicate the peripheral portion of the dorsal retina. c, presumptive corneal ectoderm; d, dorsal retina; le, lens; m, periocular mesenchyme; mu, muscle; o, optic nerve; rpe, retinal pigment epithelium; v, ventral retina. Scale bar in I: 40 µm for A-C; 50 µm for D-F; 80 µm for G-I.