Fig. 1. HhN does not influence Sxl nuclear entry. A wing disc stained for Ci (red; A,C,D), Sxl (green; B,C,E) and ß-gal (blue; D,E). ß-Gal marks the actin>GAL4 clones expressing HhN (Hh with only the palmitoyl modification). The disc was treated with 100 ng/ml LMB for 3 hours. Full-length Ci is detected anterior to the AP boundary (e.g. arrow in A). In the ß-gal-positive region where HhN levels are maximal (arrowheads) there is a reduction of full-length Ci, but no effect on Sxl nuclear entry (B,E; brighter, more punctate signal) (Horabin et al., 2003). Insets in A,B enlarge the areas indicated by arrowheads. (C) Overlay of Sxl and Ci; (D) overlay of Ci and ß-gal; (E) overlay of Sxl and ß-gal. Scale bar: 40 µm. Anterior towards the left, ventral is towards the top. Confocal images throughout.