Fig. 3. Sxl enhances Hh signaling. Discs were treated with 150 ng/ml LMB for 3 hours and antibody incubations were carried out in the same dish (sexed using Sxl signal). (A-F) Wild-type male (A-C) and female (D-F) discs stained for Ci (red) and Sxl (green). The Ci signal in males is relatively diffuse in contrast to females where it is brighter and more discrete, indicative of nuclear localization. (G-I) Male discs expressing Sxl in dorsal compartment by ap-GAL4. The level of Sxl protein is low (arrowhead in H) compared with endogenous signal of females; Ci levels are increased in the dorsal half (arrowhead in G) relative to the ventral half (arrow in G) of the disc. (J-L) Female disc expressing Sxl in dorsal compartment by ap-GAL4. The Ci signal shows an enhancement even in females (arrowhead marks the dorsoventral boundary at the AP boundary). Bracket in I,L indicates expansion of the dorsal half of the disc. Scale bars: 20 µm. Anterior is towards the left, ventral is towards the top. Confocal images throughout.