Fig. 7. Dpp signaling target pMad is enhanced by Sxl. (A-C) Wild type; (D-F) male and female (G-I) discs expressing Sxl in the dorsal compartment stained for ß-gal (red; reports dpp expression though dpp-lacZ) and pMad (green; reports activation of Dpp signaling). (C,F,I) Merged images. Below the dorsoventral boundary of the wing pouch (arrowheads) is a widened zone of Dpp expression (D-I) and corresponding widening of pMad compared with the ventral half. Scale bars: in C, 20 µm for A-C; in F, 20 µm for D-F; in I, 20 µm for G-I. Anterior is towards the left, ventral is towards the top. Confocal images throughout.