Fig. 3. Detailed visualization of heart cell migration. (A-H) Transgenic Mesp-GFP embryos. (A) Early tailbud embryo, 12 hours post-fertilization (hpf), ventral view. (B) Tailbud embryo (14 hpf), lateral view. (C) Ventrolateral view of a slightly more advanced embryo (15 hpf). Note that caudal siblings are elongated with punctate accumulations of GFP. (D) Late tailbud embryo (17 hpf), including magnified views of the two lineages. (E-G) High magnification ventral views of three sets of TVCs from progressively older embryos (17-18 hpf). (H) Ventrolateral view of an embryo at 18 hpf. All confocal images display GFP fluorescence in green and are shown with the anterior to the left. In A,C and H, the red channel displays phalloidin staining (Alexa-fluor 647, Molecular Probes). In B and D, the red channel displays auto-fluorescence.