Fig. 4. Activator Mesp fusion protein blocks heart cell migration. (A-C)
Transgenic Mesp-GFP embryos. (D-F) Embryos co-electroporated
with Mesp-VP16. (A,D) Tailbud embryos (
14 hpf) displayed
laterally. (B,E) Late tailbud embryos (
18 hpf) displayed laterally. (C,F)
Late tailbud embryos (
18 hpf) displayed ventrolaterally. Embryos are
oriented with anterior to the left; GFP fluorescence is in green, phalloidin
staining in red. (G,H) Still shots from the movies of transgenic
Mesp-VP16 juveniles (Movies 1 and 2 in the supplementary material,
respectively). Red arrowhead marks ectopic beating heart tissue; arrow marks
the site of tail resorption. The site of typical heart formation is marked by
a black arrowhead. E, endostyle; S, stomach. Lateral views with anterior to
the left.