Fig. 3. Rho1 localization during embryogenesis. Rho1 (red). DE-Cad (green). (A-C) Cross-sections, cellularization. Wild type (A,B). p120 (C). (A) Rho1 enriched at furrow canals (A, blue arrowhead). DE-Cad accumulates at basal junctions (A, yellow arrowhead) and apical AJs (A, white arrowhead). (B,C) Loss of p120 (C) does not alter Rho1 localization. (D-E) Cross-section, posterior midgut (E, close-up). Rho1 in basal puncta in invaginating midgut cells (arrowhead). DE-Cad (green, E) at sites of apical constriction. (F) Ventral furrow. DE-Cad accumulates at AJs of apically constricting cells (arrowheads) while Rho1 does not. (G-I) Cross sections, stage 8. Rho1 accumulates where ectodermal and mesodermal cells meet (G,H, arrowheads). H, close-up of G. (I) ßPS1 integrin. (J-L) Basolateral sections. Stages 5 (J), 9 (K), 15 (L). Rho1 cortical enrichment decreases. (M-N'') Cross-sections, stage 15 hindgut. Wild type (M). p120 mutant (N). Rho1 accumulates basally (arrowheads). DE-Cad accumulates in apical AJs. Scale bars: 20 µm.