Fig. 4. Neither p120 nor core AJ components are essential for Rho1 localization during dorsal closure. (A,B) Wild type. DE-Cad (A,B) versus Rho1 (A',B') in apical (A,A') and basolateral (B,B') sections. (A,A') Apically, DE-Cad (A) accumulates at AJs. Leading edge (arrowhead). Rho1 (A') is at low levels in the cytoplasm and is not enriched at the leading edge (arrowhead). (B,B') Basally, DE-Cad (B) accumulates at AJs in segmental grooves (arrowhead). Rho1 (B') levels are higher and it is cortically enriched (arrowhead). (C,C') p120 mutant. Rho1 is not elevated at the leading edge (arrowheads, C' versus A'). (D,D') Apical RhoGEF2. Arrowheads, leading edge. (E-G) Basolateral Rho1 (arrowhead) is similar in wild type (E), arm^YD35 (F), or shg^R69 zygotic mutants (G). (G') DE-Cad in a portion of G. (H,H') RhoGEF2 is enriched basolaterally and is more cortical than Rho1 (H' versus B') at both amnioserosal (arrow) and epidermal cell (arrowhead) boundaries. (I-M'') Rho1 in shg^R69 mutant follicle cell clones. (I-K') GFP, green; Arm, blue; DE-Cad, red. (I) Cross-section, early egg chamber. shg^R69 mutant clone (bracket) indicated by lack of GFP. Arm accumulates at AJs at this stage despite loss of DE-Cad. (J-K') Cross-section (J,J'), grazing section (K,K'), later egg chamber. shg^R69 mutant clone (bracket). Arm and DE-Cad are severely reduced. (L-M'') Grazing section, similar stage egg chamber as J,K. GFP, green; Rho1, blue; DE-Cad, red. (L) Rho1 accumulates normally in shg^R69 clone. (M,M'') Close-up of L. Scale bars: 20 µm in A; 50 µm in I-L,M''.