Fig. 5. Increased Xnr expression in Zic2-depleted embryos is restricted to vegetal cells. (A) Xnr5 in situ hybridization at stages 9 (upper panel) and 10.5 (lower panel). Dorsal is oriented towards the top of the figure. Xnr5 is expressed in dorsal vegetal cells of uninjected and Zic2-depleted embryos. Xnr5 expression at stage 10.5 is undetectable in control embryos (0/11) but is present ventrally in Zic2-depleted embryos (8/13; arrow). (B) Expression of Xnr genes and Xnr target genes in dorsal (D) and ventral (V) halves at stage 10.25. Uninjected (Uninj.) 10.25 and Zic2-depleted (Zic2-) 10.25 are intact embryos. –RT, stage 10.25 sample processed in the absence of reverse transcriptase. All genes shown are expressed ventrally. (C) Expression of Xnr genes and Xnr target genes in equatorial (Eq) and vegetal base (Bs) explants.