Fig. 7. Zic2 antagonizes VegT regulation of Xnr expression. (A) Expression of Xnr1, Xnr3 and Xnr5 in whole embryos at stage 10.5. Un, uninjected; Zic2-, 5 ng 10MP; VegT MO, 18 ng morpholino oligo. (B) Expression of Xnr5 in uninjected and Zic2-depleted embryos in the absence or presence of VegT mRNA (200 pg). Oligos were injected vegetally in A and Zic2 oligo and VegT RNA were injected animally in B. (C) Zic2 does not inhibit VegT in animal caps. Zic2 mRNA (500 pg), VegT mRNA (250 pg) or a combination of the two, were injected at the two-cell stage. Animal caps were dissected at the early gastrula stage and cultured for 2 hours.