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Fig. 3. Expression of MAD1 results in expanded Drosophila SRF expression as a result of reduced rhomboid transcription. (A) Wild-type pupal wing disc stained for Drosophila SRF expression. Anterior is towards the top and distal to the left. L1-L5 primordia are indicated. (B) UAS.Mad/ptc.Gal4 has expanded regions without Drosophila SRF corresponding to L3/L4. (C) UAS.Mad12/69B.Gal4 appears wild type. (D) UAS.Mad1/69B.Gal4 with expanded regions of Drosophila SRF that limit the extent of L3/L4. (E) Wild-type pupal wing disc stained for rhomboid (rho) transcripts. L3/L4/L5 primordia are indicated. rho expression in L1/L2 is not yet fully developed at this stage; note the difference in the visibility of L1/L2 versus L3/L4/L5 in A. (F) UAS.Mad1/69B.Gal4 with reduced rho expression. Expression in L4 is severely reduced and distal truncations in L3/L5 are visible.