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Fig. 7. Rescue of cardioblast specification by forced expression of cardiogenic regulators. Shown are stage 14-15 embryos double labeled for mid mRNA and Tin protein to monitor cardioblast (arrows) specification. (A) Wild type showing normal mid and Tin expression in cardioblasts, as well as Tin in pericardial cells. (B) In homozygous DocA mutants, very few cardioblasts are detectable, although Tin+ Eve-PCs are still present. (C-F) DocA mutants expressing various UAS-rescue constructs under control of the dorsal mesoderm driver tinD-GAL4. (C) Cardioblast formation is moderately rescued by UAS-pnr. (D) In DocA mutants with tinD-driven tin, only few additional cardioblasts are formed compared with DocA mutants without any rescue construct. (E) UAS-Doc2 causes efficient rescue of cardioblast specification in a DocA mutant background. (F) UAS-Doc3 shows a moderate rescuing activity.