Fig. 1. Phenotype of daedalus mutant embryos. (A,B) Dorsal views of live
5-day-old sibling (A) and dae mutant (B) larvae, anterior towards the
left. dae mutants lack pectoral fins, but appear otherwise normal.
(C,D) Alcian Blue staining of the pectoral fin endoskeleton. Wild-type
endoskeleton (C) consists of a pectoral girdle, postcoracoid process and
endoskeletal disk attached to the cleithrum. dae mutants (D) retain
only a dysmorphic pectoral girdle and cleithrum. (E,F) Transverse cryosections
of 40 hpf mutant and sibling embryos stained with Methylene Blue. Mutant
embryos (F) have a smaller undifferentiated fin bud, without an apical ridge
(ar, arrow), when compared with siblings (E). ed, endoskeletal disc; pp,
postcoracoid process; sc, scapulocoracoid.