Fig. 5. Drosophila Tral and Me31B form a RNA-dependent complex and colocalize in the germline. (A) RNA-dependent interaction between Drosophila Tral (CAR-1 ortholog) and Me31B (CGH-1 ortholog). Extracts from Drosophila ovaries were immunoprecipitated with a Me31B antibody (Nakamura et al., 2004), and analyzed for the presence of Tral by western blotting. Co-immunoprecipitation of Tral with Me31B (lane 3) was abolished by RNase A treatment (lane 4). Lane 2 shows protein extract incubated with control IgG. (B-D) Extensive colocalization (D) of Me31B (B) and Tral (C) in cytoplasmic granules in the Drosophila germline. A stage-7 egg chamber expressing GFP-Me31B was stained with a Tral-specific antibody.